Gene amplification is one of the major mechanisms of oncogene activation in tumorigenesis. Currently, however, direct identification of amplified genes in cancer remains difficult. The most important ...

To identify differentially expressed genes and scan candidate molecular markers in nasopharyngeal carcinoma (NPC). We constructed four subtracted cDNA libraries using suppression subtractive ...

Messenger RNA (mRNA) is isolated from the sample and converted into complimentary DNA (cDNA) The complimentary DNA is then labeled with a fluorescent dye The fluorescently labeled complimentary DNA is ...

Researchers have invented a technique in which DNA assays -- the key to personalized medicine -- can be read and evaluated with no need of elaborate chemical labeling or sophisticated instrumentation.

For 20 years, DNA microarrays have been the go-to method for revealing patterns of gene expression. But as the price of next-generation sequencing continues to fall, RNA sequencing (RNA-seq) has ...

We reviewed the options for the manufacture and use of DNA microarrays in the original Chipping Forecast 1 in 1999. Since then, the number of companies that produce microarrays equipment and reagents ...

To prevent outbreaks of water- or food-borne illnesses, scientists need rapid, sensitive methods to detect pathogenic bacteria such as Escherichia coli, Salmonella enterica, and Campylobacter jejuni.